Liu Hu, a native of Xinji, Hebei Province, is a pre-appointed assistant professor and doctoral supervisor at the Institute of Biochemical Engineering, School of Chemistry and Chemical Engineering, Beijing Institute of Technology. Her research interests include enzyme Engineering, protein engineering, synthetic biology, and research on intelligent design of enzyme molecules and efficient assembly of proteins; host and participate in several National Natural Science Foundation projects, host two national key research and development program sub-topics, and serve as the editorial board member of the Biochemical Engineering Journal. He has been responsible for teaching courses such as "Protein Drug Design and Synthesis", "Biological Drug Separation Technology" and "Biochemistry B", and guided undergraduates to win the 2022 National Genetic Engineering Machine Competition and won the team Silver award. At present, there are 6 doctoral students and master students under my supervision. We welcome students who are practical, diligent and interested in scientific research to join our team!

Homologous oligomerases are a class of enzyme molecules composed of the same subunits, whose oligomerization structure is essential for catalytic function. The oligomerization structure of homologous oligomerase was artificially regulated by protein structure analysis and computer simulation, and its oligomerization structure evolution and structure-activity relationship were studied. The oligomerization structure of homologous oligomerase was modified by protein engineering, and a new method of oligomerization regulation was explored to improve the catalytic efficiency of the enzyme, and artificial monomer enzymes or polymerase molecules were designed to meet the application requirements. By means of genetic engineering and protein engineering, the efficient assembly strategy of proteins, nucleic acids and other biological macromolecules, as well as the adaptive assembly between proteins and cells, surface display technology, etc., are studied to explore their applications as biological materials in food safety, toxin degradation, enzyme immobilization and drug release.

2010/09-2015/06, School of Chemical Engineering, Tianjin University, PhD in Biochemistry
2006/09-2010/07, School of Chemical Engineering, Hebei University of Technology, B.S.

07/2019 - Present, Beijing Institute of Technology, School of Chemistry and Chemical Engineering, Special Associate Researcher
2016/06-2019/06, Beijing Institute of Technology, School of Life Sciences, Team Post Doctor

Main Research Projects
1. Self-assembly enzyme design and functional regulation based on domain exchange, National Natural Science Foundation of China, 2023-2026, 540,000, Host.
2. Intelligent design and functional regulation of key enzymes of metabolic pathway, National Key Research and Development Plan "Synthetic Biology" key project, 2022-2027, 600,000 yuan, sub-project leader.
3. Structure-activity relationship of immobilized enzyme and design principle of universal carrier, Key project of National Key Research and Development Plan "Green Bio-manufacturing", 2021-2024, 500,000 yuan, sub-project leader.
4. Catalytic structure evolution of β-glucuronidase homologous oligomers to monomers, National Natural Science Foundation of China Youth Fund Project, 2018-2019, 280 million, Host.
5. Enhancement of transmembrane transport and metabolic mass transfer during Microbial synthesis of terpene compounds, Key Project of National Natural Science Foundation of China, 2018-20223.2 million yuan, participated.
6. Study on stress resistance omics and multiple stress resistance gene circuits of Saccharomyces Cerevisiae, National Natural Science Foundation of China Youth Fund Project, 2019-2021, 300,000, participated.
7. Beijing Institute of Technology Young Teachers Academic Start-up Project, Enzyme Self-assembly Design and Catalytic Function Regulation, 2019-2022, 400,000, host.
Representative Papers
1. Wang Qibin, Lu Dingyuan, Liu Hu*, Li Chun*, Tetramerization of GH2β ‑Glucuronidases is Essential for Catalyzing the Hydrolysis of the Large Substrate Glycyrrhizin, Journal of Agricultural and Food Chemistry, 2022, 70(51): 16286–16297.
2. Chen Xinyi, Li Chun, Liu Hu*, Enhanced Recombinant Protein Production Under Special Environmental Stress, Frontiers in Microbiology, DOI: 10.3389 / fmicb. 2021.630814. < br > 3. Liu Hu, Cao Mingming, Wang Ying, Lv Bo and Li Chun*. Bioengineering oligomerization and monomerization of enzymes: learning from natural evolution to matching the demands for industrial applications. Critical Reviews in Biotechnology, 2020, 40(2):231-246.
4. Hu Liu#, Jingjing Fan#, Chen Wang, Chun Li, Xiaohong Zhou*. Enhanced β-Amyrin Synthesis in Saccharomyces cerevisiae by Coupling An Optimal Acetyl-CoA Supply Pathway. Journal of Agricultural and Food Chemistry, 2019, 67(13), 3723-3732.
5. Hu Liu, Xiaoyan Dong, Yan Sun*. Enhanced Protein Adsorption and Facilitated Refolding of Like-Charged Protein with Highly Charged Silica Nanoparticles Fabricated by Sequential Double Modifications. Langmuir, 2015, 31 (2) 655– 658.
6. Hu Liu, Xiaoyan Dong, Yan Sun*. Grafting iminodiacetic acid on silica nanoparticles for facilitated refolding of like-charged protein and its metal-chelate affinity purification. Journal of Chromatography A, 2016, 1429, 277– 283.
7. Hu Liu#, Wenjie Du#, Xiaoyan Dong, Yan Sun*. Integrative refolding and purification of histidine-tagged protein by like-charge facilitated refolding and metal-chelate affinity adsorption. Journal of Chromatography A, 2014, 1344, 59– 65.
8. Hu Liu, Xiaoyan Dong, Yan Sun*. Peptide disulfide RKCGCFF facilitates oxidative protein refolding by mimicking protein disulfide isomerase. Biochemical Engineering Journal, 2013, 79, 29– 32.
9. Wentao Sun, Haijie Xue, Hu Liu, Bo Lv, Yang Yu,* Ying Wang*, Meilan Huang, and Chun Li*, Controlling Chemo- and Regioselectivity of a Plant P450 in Yeast Cell toward Rare Licorice Triterpenoid Biosynthesis, ACS Catalysis, 2020, 10, 4253−4260.
10. Teng Zhang, Hu Liu, Bo Lv* and Chun Li*, Regulating Strategies for Producing Carbohydrate Active Enzymes by Filamentous Fungal Cell Factories, The Frontiers in Bioengineering and Biotechnology, DOI: 10.3389 / fbioe. 2020.00691. < br > 11. Liu Hu, Li Chun *. Oligomerization structure and catalytic stability of enzymes. Journal of Chemical Engineering, 2018, 69(1): 352-362.
12. Zhang Zhenghui, Cao Mingming, Li Jun, Li Chun, Liu Hu *. Strategies and applications of efficient protein secretion by microorganisms. Chemical Industry Progress, 2018, 37(8): 3129-3137. (in Chinese)
13. Zhi-Qiang Jiang, Xiao-Yan Dong, Hu Liu, Yongjian Wang, Lei Zhang, Yan Sun*. Multifunctionality of self-assembled nanogels of curcumin-hyaluronic acid conjugates on inhibiting amyloid beta-protein fibrillation and cytotoxicity [J], Reactive and Functional polymers, 2016, 104: 22–29.
14. Liu Xiaochen, Liu Hu, Zhang Liang, Li Chun *. Enzymatic glycosylation and its functions in cell metabolism. Chinese Journal of Bioengineering. 2018, 38(1) 10-22.
Authorized patent
1. Dong Xiaoyan, Liu Hu, Yu Linling, Sun Yan. A metal chelating nano medium and a preparation method thereof and a method for Enhancing Inclusion body protein renaturation and integrated purification. 2017.09.29, China, ZL 201510591215. X
2. Li Jun, YU Liping, Liu Hu, Li Chun. A method and application for improving cell viability and multiple stress resistance of Saccharomyces Cerevisiae. 2021.09.17, China, ZL 201710573663.6
3. Li Chun, Liu Xiaochen, Zhang Liang, Ni Jiangping, Liu Hu. Enzymatic synthesis of 3-O-glucose-glycyrrhetinic acid. 2022.02.18, China, ZL 201710950480.1