USP10 exacerbates neointima formation by stabilizing Skp2 protein in vascular smooth muscle cells

Xiaohong Xia; Xiaolin Liu; Renjie Chai; Qiong Xu; Zhenyu Luo; Jielei Gu; Yangshuo Jin; Tumei Hu; Cuifu Yu; Bijun Du; Hongbiao Huang; Wenchao Ou; Shiming Liu; Ningning Liu

doi:10.1016/j.jbc.2021.101258

USP10 exacerbates neointima formation by stabilizing Skp2 protein in vascular smooth muscle cells

Xiaohong Xia, Xiaolin Liu, Renjie Chai, Qiong Xu, Zhenyu Luo, Jielei Gu, Yangshuo Jin, Tumei Hu, Cuifu Yu, Bijun Du, Hongbiao Huang, Wenchao Ou, Shiming Liu^*, Ningning Liu^*

^*Corresponding author for this work

Guangzhou Medical College

Research output: Contribution to journal › Article › peer-review

11 Citations (Scopus)

Abstract

The underlying mechanism of neointima formation remains unclear. Ubiquitin-specific peptidase 10 (USP10) is a deubiquitinase that plays a major role in cancer development and progression. However, the function of USP10 in arterial restenosis is unknown. Herein, USP10 expression was detected in mouse arteries and increased after carotid ligation. The inhibition of USP10 exhibited thinner neointima in the model of mouse carotid ligation. In vitro data showed that USP10 deficiency reduced proliferation and migration of rat thoracic aorta smooth muscle cells (A7r5) and human aortic smooth muscle cells (HASMCs). Mechanically, USP10 can bind to Skp2 and stabilize its protein level by removing polyubiquitin on Skp2 in the cytoplasm. The overexpression of Skp2 abrogated cell cycle arrest induced by USP10 inhibition. Overall, the current study demonstrated that USP10 is involved in vascular remodeling by directly promoting VSMC proliferation and migration via stabilization of Skp2 protein expression.

Original language	English
Article number	101258
Journal	Journal of Biological Chemistry
Volume	297
Issue number	5
DOIs	https://doi.org/10.1016/j.jbc.2021.101258
Publication status	Published - 1 Nov 2021
Externally published	Yes

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title = "USP10 exacerbates neointima formation by stabilizing Skp2 protein in vascular smooth muscle cells",

abstract = "The underlying mechanism of neointima formation remains unclear. Ubiquitin-specific peptidase 10 (USP10) is a deubiquitinase that plays a major role in cancer development and progression. However, the function of USP10 in arterial restenosis is unknown. Herein, USP10 expression was detected in mouse arteries and increased after carotid ligation. The inhibition of USP10 exhibited thinner neointima in the model of mouse carotid ligation. In vitro data showed that USP10 deficiency reduced proliferation and migration of rat thoracic aorta smooth muscle cells (A7r5) and human aortic smooth muscle cells (HASMCs). Mechanically, USP10 can bind to Skp2 and stabilize its protein level by removing polyubiquitin on Skp2 in the cytoplasm. The overexpression of Skp2 abrogated cell cycle arrest induced by USP10 inhibition. Overall, the current study demonstrated that USP10 is involved in vascular remodeling by directly promoting VSMC proliferation and migration via stabilization of Skp2 protein expression.",

author = "Xiaohong Xia and Xiaolin Liu and Renjie Chai and Qiong Xu and Zhenyu Luo and Jielei Gu and Yangshuo Jin and Tumei Hu and Cuifu Yu and Bijun Du and Hongbiao Huang and Wenchao Ou and Shiming Liu and Ningning Liu",

note = "Publisher Copyright: {\textcopyright} 2021 THE AUTHORS.",

year = "2021",

month = nov,

day = "1",

doi = "10.1016/j.jbc.2021.101258",

language = "English",

volume = "297",

journal = "Journal of Biological Chemistry",

issn = "0021-9258",

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TY - JOUR

T1 - USP10 exacerbates neointima formation by stabilizing Skp2 protein in vascular smooth muscle cells

AU - Xia, Xiaohong

AU - Liu, Xiaolin

AU - Chai, Renjie

AU - Xu, Qiong

AU - Luo, Zhenyu

AU - Gu, Jielei

AU - Jin, Yangshuo

AU - Hu, Tumei

AU - Yu, Cuifu

AU - Du, Bijun

AU - Huang, Hongbiao

AU - Ou, Wenchao

AU - Liu, Shiming

AU - Liu, Ningning

PY - 2021/11/1

Y1 - 2021/11/1

N2 - The underlying mechanism of neointima formation remains unclear. Ubiquitin-specific peptidase 10 (USP10) is a deubiquitinase that plays a major role in cancer development and progression. However, the function of USP10 in arterial restenosis is unknown. Herein, USP10 expression was detected in mouse arteries and increased after carotid ligation. The inhibition of USP10 exhibited thinner neointima in the model of mouse carotid ligation. In vitro data showed that USP10 deficiency reduced proliferation and migration of rat thoracic aorta smooth muscle cells (A7r5) and human aortic smooth muscle cells (HASMCs). Mechanically, USP10 can bind to Skp2 and stabilize its protein level by removing polyubiquitin on Skp2 in the cytoplasm. The overexpression of Skp2 abrogated cell cycle arrest induced by USP10 inhibition. Overall, the current study demonstrated that USP10 is involved in vascular remodeling by directly promoting VSMC proliferation and migration via stabilization of Skp2 protein expression.

AB - The underlying mechanism of neointima formation remains unclear. Ubiquitin-specific peptidase 10 (USP10) is a deubiquitinase that plays a major role in cancer development and progression. However, the function of USP10 in arterial restenosis is unknown. Herein, USP10 expression was detected in mouse arteries and increased after carotid ligation. The inhibition of USP10 exhibited thinner neointima in the model of mouse carotid ligation. In vitro data showed that USP10 deficiency reduced proliferation and migration of rat thoracic aorta smooth muscle cells (A7r5) and human aortic smooth muscle cells (HASMCs). Mechanically, USP10 can bind to Skp2 and stabilize its protein level by removing polyubiquitin on Skp2 in the cytoplasm. The overexpression of Skp2 abrogated cell cycle arrest induced by USP10 inhibition. Overall, the current study demonstrated that USP10 is involved in vascular remodeling by directly promoting VSMC proliferation and migration via stabilization of Skp2 protein expression.

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DO - 10.1016/j.jbc.2021.101258

M3 - Article

C2 - 34599966

AN - SCOPUS:85118916703

SN - 0021-9258

VL - 297

JO - Journal of Biological Chemistry

JF - Journal of Biological Chemistry

IS - 5

M1 - 101258

ER -

USP10 exacerbates neointima formation by stabilizing Skp2 protein in vascular smooth muscle cells

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