TY - JOUR
T1 - Protease-resistant core form of Bacillus thuringiensis Cry1ie
T2 - Monomeric and oligomeric forms in solution
AU - Guo, Shuyuan
AU - Zhang, Yancai
AU - Song, Fuping
AU - Zhang, Jie
AU - Huang, Dafang
PY - 2009/10
Y1 - 2009/10
N2 - Cry1Ie is encoded by cry1Ie, a gene that is silent in Bacillus thuringiensis strains but can be over-expressed in Escherichia coli. A protease-resistant core form of Cry1Ie with a molecular weight of approx. 55 kDa was found among the products of trypsin digestion. The oligomer and monomer of the protease-resistant core form were purified separately. The oligomer fraction contained a small quantity of dimer and a large amount of aggregates larger than tetramers. The 50% lethal concentrations (LC50) of the full-length Cry1Ie and both the monomer and oligomer fractions of the protease-resistant core form against Plutella xylostella were 14, 21 and 1400 μg/ml, respectively.
AB - Cry1Ie is encoded by cry1Ie, a gene that is silent in Bacillus thuringiensis strains but can be over-expressed in Escherichia coli. A protease-resistant core form of Cry1Ie with a molecular weight of approx. 55 kDa was found among the products of trypsin digestion. The oligomer and monomer of the protease-resistant core form were purified separately. The oligomer fraction contained a small quantity of dimer and a large amount of aggregates larger than tetramers. The 50% lethal concentrations (LC50) of the full-length Cry1Ie and both the monomer and oligomer fractions of the protease-resistant core form against Plutella xylostella were 14, 21 and 1400 μg/ml, respectively.
KW - Bacillus thuringiensis
KW - Cry1Ie protein
KW - Oligomer
KW - Protease-resistant core form
UR - http://www.scopus.com/inward/record.url?scp=70350300627&partnerID=8YFLogxK
U2 - 10.1007/s10529-009-0078-2
DO - 10.1007/s10529-009-0078-2
M3 - Article
C2 - 19590825
AN - SCOPUS:70350300627
SN - 0141-5492
VL - 31
SP - 1769
EP - 1774
JO - Biotechnology Letters
JF - Biotechnology Letters
IS - 11
ER -