@inproceedings{15a113345dc14bd5b9b986a4554dbc1d,
title = "Generation of rotational flow for formation of spheroid by using microfluidics device",
abstract = "Spheroid is important in the field of tissue engineering because it has a hole enabling efficient supply of oxygen and growth factors, and a vessel-like geometry is made by piling up it. But it is difficult to say that formation processes of toroidal-like spheroid is efficient, as most of them has done manually. In this study, we wish to suggest utility and possibility of applying microfluidics for formation of toroidal-like spheroid. The concept of our method is that cells are condensed by rotational flow in the microchannel and dielectrophoresis force. Some types of microchannels to generate rotational flow were designed with computer aided design (CAD) and analyzed with fluid analysis software. The superior microchannel was made, and NIH3T3 mouse embryo fibroblast cells suspension was flowed to it. As flow rates were changed, cells were rotated the microchannel just as our concept, and about 30% of them remained. This result support utility of our concept; toroidal-like spheroid can be formed under forces of rotational flow and dielectrophoresis.",
keywords = "MEMS, Microfluidics, Spheroid",
author = "Masaru Kojima and Mitsuhiro Horade and Hirochika Takai and Kenichi Ohara and Tamio Tanikawa and Kazuto Kamiyama and Yasushi Mae and Tatsuo Arai",
note = "Publisher Copyright: {\textcopyright} 2015 IEEE.; 12th IEEE International Conference on Mechatronics and Automation, ICMA 2015 ; Conference date: 02-08-2015 Through 05-08-2015",
year = "2015",
month = sep,
day = "2",
doi = "10.1109/ICMA.2015.7237629",
language = "English",
series = "2015 IEEE International Conference on Mechatronics and Automation, ICMA 2015",
publisher = "Institute of Electrical and Electronics Engineers Inc.",
pages = "1042--1047",
booktitle = "2015 IEEE International Conference on Mechatronics and Automation, ICMA 2015",
address = "United States",
}