Effect of N-terminal and C-terminal deletion of Cry1Ie of Bacillus thuringiensis on its expression and purification

Huicong Shen; Chunlu Zhang; Shuyuan Guo; Jie Zhang; Fuping Song

doi:10.1109/ICBBE.2009.5162705

Effect of N-terminal and C-terminal deletion of Cry1Ie of Bacillus thuringiensis on its expression and purification

Huicong Shen, Chunlu Zhang, Shuyuan Guo^*, Jie Zhang, Fuping Song

^*Corresponding author for this work

School of Life Science

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

Abstract

Crystal proteins are produced by the gram-positive bacterium Bacillus thuringiensis (Bt) during sporulation. The cry1Ie genes were silent in Bt strains, but can be overexpressed in Escherichia coli BL21 (DE3) in inclusion bodies, and the expressed product was toxic to Plutella xylostella, Ostrinia furnacalis, and soybean pod borer (Leguminivora glycinivorella). In order to know the N-terminal and C-terminal amino acid on the structure formation of Cry1Ie protein, four deletion mutants were constructed and the expression and purification of these mutants were studied.

Original language	English
Title of host publication	3rd International Conference on Bioinformatics and Biomedical Engineering, iCBBE 2009
DOIs	https://doi.org/10.1109/ICBBE.2009.5162705
Publication status	Published - 2009
Event	3rd International Conference on Bioinformatics and Biomedical Engineering, iCBBE 2009 - Beijing, China Duration: 11 Jun 2009 → 13 Jun 2009

Publication series

Name	3rd International Conference on Bioinformatics and Biomedical Engineering, iCBBE 2009

Conference

Conference	3rd International Conference on Bioinformatics and Biomedical Engineering, iCBBE 2009
Country/Territory	China
City	Beijing
Period	11/06/09 → 13/06/09

Keywords

Bacillus thuringienesis
Cry1Ie
Insecticidal protein
Mutant

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10.1109/ICBBE.2009.5162705

Cite this

Shen, H., Zhang, C., Guo, S., Zhang, J., & Song, F. (2009). Effect of N-terminal and C-terminal deletion of Cry1Ie of Bacillus thuringiensis on its expression and purification. In 3rd International Conference on Bioinformatics and Biomedical Engineering, iCBBE 2009 Article 5162705 (3rd International Conference on Bioinformatics and Biomedical Engineering, iCBBE 2009). https://doi.org/10.1109/ICBBE.2009.5162705

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title = "Effect of N-terminal and C-terminal deletion of Cry1Ie of Bacillus thuringiensis on its expression and purification",

abstract = "Crystal proteins are produced by the gram-positive bacterium Bacillus thuringiensis (Bt) during sporulation. The cry1Ie genes were silent in Bt strains, but can be overexpressed in Escherichia coli BL21 (DE3) in inclusion bodies, and the expressed product was toxic to Plutella xylostella, Ostrinia furnacalis, and soybean pod borer (Leguminivora glycinivorella). In order to know the N-terminal and C-terminal amino acid on the structure formation of Cry1Ie protein, four deletion mutants were constructed and the expression and purification of these mutants were studied.",

keywords = "Bacillus thuringienesis, Cry1Ie, Insecticidal protein, Mutant",

author = "Huicong Shen and Chunlu Zhang and Shuyuan Guo and Jie Zhang and Fuping Song",

year = "2009",

doi = "10.1109/ICBBE.2009.5162705",

language = "English",

isbn = "9781424429028",

series = "3rd International Conference on Bioinformatics and Biomedical Engineering, iCBBE 2009",

booktitle = "3rd International Conference on Bioinformatics and Biomedical Engineering, iCBBE 2009",

note = "3rd International Conference on Bioinformatics and Biomedical Engineering, iCBBE 2009 ; Conference date: 11-06-2009 Through 13-06-2009",

}

Shen, H, Zhang, C, Guo, S, Zhang, J & Song, F 2009, Effect of N-terminal and C-terminal deletion of Cry1Ie of Bacillus thuringiensis on its expression and purification. in 3rd International Conference on Bioinformatics and Biomedical Engineering, iCBBE 2009., 5162705, 3rd International Conference on Bioinformatics and Biomedical Engineering, iCBBE 2009, 3rd International Conference on Bioinformatics and Biomedical Engineering, iCBBE 2009, Beijing, China, 11/06/09. https://doi.org/10.1109/ICBBE.2009.5162705

Effect of N-terminal and C-terminal deletion of Cry1Ie of Bacillus thuringiensis on its expression and purification. / Shen, Huicong; Zhang, Chunlu; Guo, Shuyuan et al.
3rd International Conference on Bioinformatics and Biomedical Engineering, iCBBE 2009. 2009. 5162705 (3rd International Conference on Bioinformatics and Biomedical Engineering, iCBBE 2009).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

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T1 - Effect of N-terminal and C-terminal deletion of Cry1Ie of Bacillus thuringiensis on its expression and purification

AU - Shen, Huicong

AU - Zhang, Chunlu

AU - Guo, Shuyuan

AU - Zhang, Jie

AU - Song, Fuping

PY - 2009

Y1 - 2009

N2 - Crystal proteins are produced by the gram-positive bacterium Bacillus thuringiensis (Bt) during sporulation. The cry1Ie genes were silent in Bt strains, but can be overexpressed in Escherichia coli BL21 (DE3) in inclusion bodies, and the expressed product was toxic to Plutella xylostella, Ostrinia furnacalis, and soybean pod borer (Leguminivora glycinivorella). In order to know the N-terminal and C-terminal amino acid on the structure formation of Cry1Ie protein, four deletion mutants were constructed and the expression and purification of these mutants were studied.

AB - Crystal proteins are produced by the gram-positive bacterium Bacillus thuringiensis (Bt) during sporulation. The cry1Ie genes were silent in Bt strains, but can be overexpressed in Escherichia coli BL21 (DE3) in inclusion bodies, and the expressed product was toxic to Plutella xylostella, Ostrinia furnacalis, and soybean pod borer (Leguminivora glycinivorella). In order to know the N-terminal and C-terminal amino acid on the structure formation of Cry1Ie protein, four deletion mutants were constructed and the expression and purification of these mutants were studied.

KW - Bacillus thuringienesis

KW - Cry1Ie

KW - Insecticidal protein

KW - Mutant

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DO - 10.1109/ICBBE.2009.5162705

M3 - Conference contribution

AN - SCOPUS:72749125820

SN - 9781424429028

T3 - 3rd International Conference on Bioinformatics and Biomedical Engineering, iCBBE 2009

BT - 3rd International Conference on Bioinformatics and Biomedical Engineering, iCBBE 2009

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Y2 - 11 June 2009 through 13 June 2009

ER -

Shen H, Zhang C, Guo S, Zhang J, Song F. Effect of N-terminal and C-terminal deletion of Cry1Ie of Bacillus thuringiensis on its expression and purification. In 3rd International Conference on Bioinformatics and Biomedical Engineering, iCBBE 2009. 2009. 5162705. (3rd International Conference on Bioinformatics and Biomedical Engineering, iCBBE 2009). doi: 10.1109/ICBBE.2009.5162705

Effect of N-terminal and C-terminal deletion of Cry1Ie of Bacillus thuringiensis on its expression and purification

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