Characterization of Strip1 Expression in Mouse Cochlear Hair Cells

Shasha Zhang; Ying Dong; Ruiying Qiang; Yuan Zhang; Xiaoli Zhang; Yin Chen; Pei Jiang; Xiangyu Ma; Leilei Wu; Jingru Ai; Xia Gao; Pengjun Wang; Jie Chen; Renjie Chai

doi:10.3389/fgene.2021.625867

Characterization of Strip1 Expression in Mouse Cochlear Hair Cells

Shasha Zhang, Ying Dong, Ruiying Qiang, Yuan Zhang, Xiaoli Zhang, Yin Chen, Pei Jiang, Xiangyu Ma, Leilei Wu, Jingru Ai, Xia Gao, Pengjun Wang^*, Jie Chen^*, Renjie Chai^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

24 Citations (Scopus)

Abstract

Striatin-interacting protein 1 (Strip1) is a core component of the striatin interacting phosphatase and kinase (STRIPAK) complex, which is involved in embryogenesis and development, circadian rhythms, type 2 diabetes, and cancer progression. However, the expression and role of Strip1 in the mammalian cochlea remains unclear. Here we studied the expression and function of Strip1 in the mouse cochlea by using Strip1 knockout mice. We first found that the mRNA and protein expression of Strip1 increases as mice age starting from postnatal day (P) 3 and reaches its highest expression level at P30 and that the expression of Strip1 can be detected by immunofluorescent staining starting from P14 only in cochlear HCs, and not in supporting cells (SCs). Next, we crossed Strip1 heterozygous knockout (Strip +/−) mice to obtain Strip1 homozygous knockout (Strip1−/−) mice for studying the role of Strip1 in cochlear HCs. However, no Strip1−/− mice were obtained and the ratio of Strip +/− to Strip1+/+ mice per litter was about 2:1, which suggested that homozygous Strip1 knockout is embryonic lethal. We measured hearing function and counted the HC number in P30 and P60 Strip +/− mice and found that they had normal hearing ability and HC numbers compared to Strip1+/+ mice. Our study suggested that Strip1 probably play important roles in HC development and maturation, which needs further study in the future.

Original language	English
Article number	625867
Journal	Frontiers in Genetics
Volume	12
DOIs	https://doi.org/10.3389/fgene.2021.625867
Publication status	Published - 6 Apr 2021
Externally published	Yes

Keywords

Strip1
cochlea
expression
hair cell
inner ear

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.3389/fgene.2021.625867

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title = "Characterization of Strip1 Expression in Mouse Cochlear Hair Cells",

abstract = "Striatin-interacting protein 1 (Strip1) is a core component of the striatin interacting phosphatase and kinase (STRIPAK) complex, which is involved in embryogenesis and development, circadian rhythms, type 2 diabetes, and cancer progression. However, the expression and role of Strip1 in the mammalian cochlea remains unclear. Here we studied the expression and function of Strip1 in the mouse cochlea by using Strip1 knockout mice. We first found that the mRNA and protein expression of Strip1 increases as mice age starting from postnatal day (P) 3 and reaches its highest expression level at P30 and that the expression of Strip1 can be detected by immunofluorescent staining starting from P14 only in cochlear HCs, and not in supporting cells (SCs). Next, we crossed Strip1 heterozygous knockout (Strip +/−) mice to obtain Strip1 homozygous knockout (Strip1−/−) mice for studying the role of Strip1 in cochlear HCs. However, no Strip1−/− mice were obtained and the ratio of Strip +/− to Strip1+/+ mice per litter was about 2:1, which suggested that homozygous Strip1 knockout is embryonic lethal. We measured hearing function and counted the HC number in P30 and P60 Strip +/− mice and found that they had normal hearing ability and HC numbers compared to Strip1+/+ mice. Our study suggested that Strip1 probably play important roles in HC development and maturation, which needs further study in the future.",

keywords = "Strip1, cochlea, expression, hair cell, inner ear",

author = "Shasha Zhang and Ying Dong and Ruiying Qiang and Yuan Zhang and Xiaoli Zhang and Yin Chen and Pei Jiang and Xiangyu Ma and Leilei Wu and Jingru Ai and Xia Gao and Pengjun Wang and Jie Chen and Renjie Chai",

note = "Publisher Copyright: {\textcopyright} Copyright {\textcopyright} 2021 Zhang, Dong, Qiang, Zhang, Zhang, Chen, Jiang, Ma, Wu, Ai, Gao, Wang, Chen and Chai.",

year = "2021",

month = apr,

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doi = "10.3389/fgene.2021.625867",

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T1 - Characterization of Strip1 Expression in Mouse Cochlear Hair Cells

AU - Zhang, Shasha

AU - Dong, Ying

AU - Qiang, Ruiying

AU - Zhang, Yuan

AU - Zhang, Xiaoli

AU - Chen, Yin

AU - Jiang, Pei

AU - Ma, Xiangyu

AU - Wu, Leilei

AU - Ai, Jingru

AU - Gao, Xia

AU - Wang, Pengjun

AU - Chen, Jie

AU - Chai, Renjie

PY - 2021/4/6

Y1 - 2021/4/6

N2 - Striatin-interacting protein 1 (Strip1) is a core component of the striatin interacting phosphatase and kinase (STRIPAK) complex, which is involved in embryogenesis and development, circadian rhythms, type 2 diabetes, and cancer progression. However, the expression and role of Strip1 in the mammalian cochlea remains unclear. Here we studied the expression and function of Strip1 in the mouse cochlea by using Strip1 knockout mice. We first found that the mRNA and protein expression of Strip1 increases as mice age starting from postnatal day (P) 3 and reaches its highest expression level at P30 and that the expression of Strip1 can be detected by immunofluorescent staining starting from P14 only in cochlear HCs, and not in supporting cells (SCs). Next, we crossed Strip1 heterozygous knockout (Strip +/−) mice to obtain Strip1 homozygous knockout (Strip1−/−) mice for studying the role of Strip1 in cochlear HCs. However, no Strip1−/− mice were obtained and the ratio of Strip +/− to Strip1+/+ mice per litter was about 2:1, which suggested that homozygous Strip1 knockout is embryonic lethal. We measured hearing function and counted the HC number in P30 and P60 Strip +/− mice and found that they had normal hearing ability and HC numbers compared to Strip1+/+ mice. Our study suggested that Strip1 probably play important roles in HC development and maturation, which needs further study in the future.

AB - Striatin-interacting protein 1 (Strip1) is a core component of the striatin interacting phosphatase and kinase (STRIPAK) complex, which is involved in embryogenesis and development, circadian rhythms, type 2 diabetes, and cancer progression. However, the expression and role of Strip1 in the mammalian cochlea remains unclear. Here we studied the expression and function of Strip1 in the mouse cochlea by using Strip1 knockout mice. We first found that the mRNA and protein expression of Strip1 increases as mice age starting from postnatal day (P) 3 and reaches its highest expression level at P30 and that the expression of Strip1 can be detected by immunofluorescent staining starting from P14 only in cochlear HCs, and not in supporting cells (SCs). Next, we crossed Strip1 heterozygous knockout (Strip +/−) mice to obtain Strip1 homozygous knockout (Strip1−/−) mice for studying the role of Strip1 in cochlear HCs. However, no Strip1−/− mice were obtained and the ratio of Strip +/− to Strip1+/+ mice per litter was about 2:1, which suggested that homozygous Strip1 knockout is embryonic lethal. We measured hearing function and counted the HC number in P30 and P60 Strip +/− mice and found that they had normal hearing ability and HC numbers compared to Strip1+/+ mice. Our study suggested that Strip1 probably play important roles in HC development and maturation, which needs further study in the future.

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KW - cochlea

KW - expression

KW - hair cell

KW - inner ear

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VL - 12

JO - Frontiers in Genetics

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ER -

Characterization of Strip1 Expression in Mouse Cochlear Hair Cells

Abstract

Keywords

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